Bacteria require iron for survival and have developed elaborate iron-scavenging mechanisms. Our goal in the Lamb group is to understand the enzymes that produce small molecules called siderophores – one iron-scavenging mechanism of these bacteria. A student completing a Beckman Scholar project will choose a siderophore biosynthetic enzyme to clone for overexpression in E. coli, develop an overproduction and purification strategy for the enzyme, and design a crystallization protocol for the enzyme to work on independently. Upon crystallization, the student will assist in remote diffraction data collection at the Stanford Synchrotron Radiation Light Source and work toward structure solution. The student will establish an assay to detect enzymatic activity and conduct steady-state analyses of the enzyme. Our scholars will also learn protein purification, enzyme analysis and structure determination, and they will be part of a dynamic group of research associates, graduate students and undergraduates. As such, our undergraduate colleagues are expected to present progress at weekly group meetings and to share in the responsibility of general lab duties. They also write monthly reports on progress, present their research at local and regional conferences, and aid in the preparation of manuscripts for publication.